Year: 2022 | Month: February | Volume 12 | Issue 1
Flow Cytometric Evaluation of Acrosomal integrity of Buffalo Spermatozoa at Different Stage of Cryopreservation using Different Extenders
Satish
G.N. Purohit
R.K. Sharma
Pradeep Kumar
A. Jerome
Devender Kumar
Renu Bala
Nisha Verma
Jayesh Vyas
J.S. Mehta
DOI:0.30954/2277-940X.01.2022.9
Abstract:
With an objective to evaluate the value of different extenders semen was collected from Murrah buffalo bulls (n=6) by artificial vagina and subjected to dilution using different extenders viz Tris egg yolk citrate (TEYC), LDL, tocopherol (TEYC with 1 mg/mL tocopherol) and sericin (TEYC with 0.25% sericin) based extenders. Semen was cryopreserved and sperm with intact acrosome were evaluated by flow cytometry immediately after dilution, at equilibration stage and after cryopreservation. There were non-significant differences in the proportion of live sperm with intact acrosome (frozen-thawed semen) for all the diluters studied except for buffalo bull number 5333 which evidenced significantly lower (P>0.05) proportion of live sperm with intact sperm in the tocopherol based extender. The overall percent live sperm with intact sperm was significantly higher (P<0.001) for LDL based extender as compared to TEYC, tocopherol, and sericin extenders at the equilibration stage and in frozen-thawed semen. It was concluded that the LDL based extender is best for cryopreservation of buffalo semen.
Highlights
- Sperms with intact acrosomes are not significantly different at three stages of cryopreservation.
- Tris supplemented with LDL yields the highest percent live sperms with intact acrosome.
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